Alternative oxidase and ATP/ADP-antiporter gene expression in transgenic Fe-SOD and Mg-SOD tobacco plants (Nicotiana tabacum) under low temperature stress

Английский. The main goal of the research presented in the article appears comparative analysis of the level of gene expression, coding alternative cyanide-resistant oxidase and ATP/ADP-antiporter in Nicotiana tabacum plants, transformed with sense gene of chloroplast Fe-SOD and mitochondrial Mn-SOD, and wild plants, suppressed low temperature stress and in post-stress period. Nucleotide sequence of direct (F) and reverse (R) primers, used for the experiment, has been presented. Conditions for PCR-analysis with primers, used for the experiment, have been described. Appearance of amplification products after dividing in agar gel has been displayed. The level of gene expression in the leaves of transgenic tobacco plants has been measured. It has been shown that the Fe-SOD transformants and especially the Mn-SOD transformants have elevated gene expression of thermogenic protein – alternative oxidase (AOXI) and ATP/ADP-antiporter (ANT) as compared to wild type under low temperature stress (+4 deg C, 22h) and during the post-stress period (+25 deg C, 24h). The results indicate an important contribution of thermogenic proteins to the forming transgenic resistance to low temperatures and during the period after termination of the stress factor.

Русский. Установлено, что трансформанты Fe-СОД и, в особенности, трансформанты Mn-СОД обладают повышенным уровнем экспресии генов белков термогенеза - альтернативной оксидазы (АОХ1) и АТФ-АДФ-антипортера (ANT) - не только при низкотемпературном стрессе (+ 4 градуса С, 22 ч), но и в постстрессовый период по сравнению с растениями дикого типа. Полученные результаты указывают на важный вклад белков термогенеза в формирование устойчивости трансгенных растений к низкой температуре, а также при выходе растений из стрессового состояния, обусловленного действием низкой температуры.