Molecular and morphological characterization of rose mutants produced via in vitro mutagenesis
2016
Aamir, S.S. | Baig, M.M.Q. | Ahmad, T. | Ghafoor, A. | Hafiz, I.A. | Abbasi, N.A. | Ali, I. | Yaseen, M.
Rose, a valuable flowering ornamental around the world, is used as cut flower, indoor potted plant, in landscape, and for extraction of essential oil. The rose varieties Rosa borboniana, Rosa centifolia and Rosa gruss-an-teplitz are tall but have small flower size, which is not desirable character as the essential oil content is dependent on flower size. To improve this trait in these rose varieties R. borboniana, R. centifolia and R. gruss-an-teplitz were treated with different doses of gamma radiation and colchicine. Surviving mutant lines were evaluated for genetic diversity from their parents using morphological and simple sequence repeat (SSR) markers. Remarkable variability was noticed for different morphological traits among the putative mutants. Maximum plant height and flower diameter was found in mutants of R. centifolia treated with colchicine. To detect genetic variation, 14 SSR primers were selected. Clusters were made unweighted pair-group method with an arithmetic average (UPGMA) analysis on the basis of close resemblances of these genotypes based on morphological characters and SSR markers. Both SSR and morphological markers showed more diversity among mutants produced colchicine. This approach is useful due to its simplicity and rapidity for identification of mutant lines which will be helpful for further improvement of desired characteristics in these mutants.
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