The effect of environmental factors on total soil DNA content and dehydrogenase activity
Wolinska, Agnieszka (The John Paul II Catholic University of Lublin, Lublin (Poland). Department of Biochemistry and Environmental Chemistry) | Stępniewska, Zofia (The John Paul II Catholic University of Lublin, Lublin (Poland). Department of Biochemistry and Environmental Chemistry) | Pytlak, Anna (The John Paul II Catholic University of Lublin, Lublin (Poland). Department of Biochemistry and Environmental Chemistry)
The aim of the study was a statistical evaluation of the impact of selected soil factors − water potential (pF), total organic carbon content (TOC) and land use − on the total DNA content and dehydrogenase activ- ity (DHA) in Mollic Gleysol. Additionally, we wanted to establish the interrelations between two of the most important biological parameters in soli: activity of intracellular dehydrogenases and total DNA content. Soil originating from the surface layer of the control site displayed higher DHA (c.a. by 57%) and DNA content (c.a. by 25%) as compared to an cultiavted meadow. Our results also indicate a significant (p <0.05) positive relation- ship between the soil DNA content and DHA. Importantly, intensive and systematical agricultural soil usage resulted in the reduction of its DHA and DNA content.
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