TILLInG and its potential for plant agriculture.
2006
Ritchie, R. D. | Nielsen, N. C.
Targeting Induced Local Lesions In Genomes (TILLInG) is a reverse genetic strategy, which combines traditional mutagenesis with high-throughput molecular biology protocols. TILLInG was first established in <i>Arabidopsis thaliana</i> when other reverse genetic strategies did not produce acceptable results. TILLInG libraries have been established in several species and more are in progress. Time must be invested in creating a good TILLInG population, such that the mutation frequency is balanced with lethality. In addition, the DNA must be stable. The number of examples of the usefulness of TILLInG libraries is continually growing. While there are some disadvantages, most can be overcome. The flexibility of TILLInG has established it as a useful strategy with great potential to advance agriculture.
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