Evaluation of Gliadin and Storage Protein Activator (Spa) in Korean Wheat Cultivars
2016
Kim, J.E., Chonbuk National University, Jeonju, Republic of Korea | Yoon, Y.M., Chonbuk National University, Jeonju, Republic of Korea | Cho, S.W., Chonbuk National University, Jeonju, Republic of Korea | Kang, C.S., National Institute of Crop Science, RDA, Wanju, Republic of Korea | Lee, J.Y., National Institute of Agricultural Sciences, RDA, Wanju, Republic of Korea | Park, C.S., Chonbuk National University, Jeonju, Republic of Korea
Genetic variations of gamma-/omega-gliadin and Spa (storage protein activator) in 40 Korean wheat cultivars were evaluated to provide genetic information for improving end-use quality in wheat breeding programs. Korean wheat cultivars were classified into 13 patterns at the Gli-1 locus based on the allelic variation using A-PAGE (acidic-polyacrylamide gel electrophoresis). Seven, five, and six alleles were identified at Gli-A1, Gli-B1, and Gli-D1 loci, respectively. Allele-specific PCR markers for gamma-gliadin corresponded to specific allele at Gli-1 loci on A-PAGE, which Gli-A1f, Gli-A1h and Gli-A1l alleles corresponded to GliA1.2, Gli-B1h and Gli-B1f alleles corresponded to GliB1.2 and Gli-D1f, Gli-D1m and Gli-D1o alleles corresponded to GliD1.1. DNA markers for gamma-45 and gamma-42 also corresponded to the gamma-gliadin patterns around 40kDa on A-PAGE, except in Sukang, Ol and Joongmo2003. However, allelic specific PCR markers for omega5-gliadin did not correspond to that of A-PAGE. Three alleles were identified at Spa-A1 locus, whereas there was no variation at Spa-B1 and Spa-D1 loci.
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