Environmental Risk Assessment and Evaluation of Vitamin E Enhanced Transgenic Soybean : Responses of Daphnia magna Fed on Vitamin E Enhanced Transgenic Soybean
2016
Oh, S.D., National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Republic of Korea | Yun, D.W., National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Republic of Korea | Sohn, S.I., National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Republic of Korea | Lee, B.K., National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Republic of Korea | Lee, K., Rural Development Administration, Jeonju, Republic of Korea | Chang, A.C., National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Republic of Korea
The vitamin E enhanced transgenic soybean was developed by introducing a perilla gamma- tocopherol methyltransferase gene (gamma-TMT) under the control of pea vicilin promoter and a selection marker, phosphinothricin acetyltransferase (PAT) gene. With regard to the potential problems of safety, the non-target organism evaluation is required as an essential element for the environmental risk assessment of genetically modified (GM) crops. We studied the effects of the vitamin E enhanced transgenic soybean feeding on survival of Daphnia magna which is commonly used as a model organism in ecotoxicological studies. The Daphnia magna was fed on vitamin E enhanced transgenic soybean and non-genetically modified (non-GM) soybean (Willams 82) at 0, 1,000, 1,800, 3,240, 5,830, 10,500 and 20,000 mg/L concentrations, respectively. The GM soybean used for the test was confirmed to have the gamma-TMT/PAT gene expression by the enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) analysis. The results showed that there was no significant differences between cumulative immobilities of Daphnia magna fed on GM soybean and non-GM soybean. The 48hr-EC50 values showed no significant differences between GM soybean (2,416 mg/L) and non-GM soybean (2,408 mg/L). The results of this study suggested that there was no significant differences in toxicity for Daphnia magna between GM soybean and non-GM counterpart.
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