Selection and Characterization of Bacillus Strains Harboring the Gene for Biogenic Amine Degradation
2017
Heo, S., Kyonggi University, Suwon, Republic of Korea | Jeong, K., Kyonggi University, Suwon, Republic of Korea | Lee, H., Kyonggi University, Suwon, Republic of Korea | Jeong, D.W., Dongduk Women's University, Seoul, Republic of Korea | Lee, J.H., Kyonggi University, Suwon, Republic of Korea
Ten Bacillus strains possessing amine oxidase activity were selected from 126 Bacillus isolates from meju and doenjang (two traditional Korean soybean foods). Among the isolates, two B. licheniformis strains (8MI05 and 8MS03) harbored the amine oxidase gene yobN. By conducting a gene search against published B. licheniformis genomes, the possession of yobN was proved to be a strain-specific property. Both strains degraded four types of biogenic amines (cadaverine, histamine, putrescine, and tyramine) supplemented in tryptic soy broth during their culture. A recombinant harboring yobN also degraded the four types of biogenic amines during cultivation. Both Bacillus strains could grow at a NaCl concentration of 14% and exhibited strain-specific protease and lipase activities.
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