Comparative evaluation of 16S rRNA gene in world-wide strains of Streptococcus iniae and Streptococcus parauberis for early diagnostic marker
Anshuman Mishra, Pusan National University, Busan, Republic of Korea | Nam, G.H., Pusan National University, Busan, Republic of Korea | Gim, J.A., Pusan National University, Busan, Republic of Korea | Seong, M., Pusan National University, Busan, Republic of Korea | Choe, Y., Pusan National University, Busan, Republic of Korea | Lee, H.E., Pusan National University, Busan, Republic of Korea | Jo, A., Pusan National University, Busan, Republic of Korea | Kim, S., Pusan National University, Busan, Republic of Korea | Kim, D.H., Pukyong National University, Busan, Republic of Korea | Cha, H.J., Kosin University, Busan, Republic of Korea | Kang, H.Y., Pusan National University, Busan, Republic of Korea | Choi, Y.H., Dongeui University, Busan, Republic of Korea | Kim, H.S., Pusan National University, Busan, Republic of Korea
Two bacterial etiological agents of the disease, Streptococcus iniae and Streptococcus parauberis has been associated with fish mortalities and heavy economic loss in all over the world. Bacterial identification based on 16S rRNA sequencing is very fast, accurate and reliable in comparison to other traditional phenotype methodologies. In this study, we investigate the usefulness of this method for diagnosis and identification of Streptococcus species. We have selected 61 phylogeographic strains of Streptococcus (34 strains of S. iniae and 27 strains of S. parauberis) and designed the universal primer against the identified most hypervariable region of the 16S rRNA gene. Our universal primer able to identify any geographical strains and offers a useful and fast alternative in a clinical laboratory under routine conditions. Based on our studies, we have developed an algorithm for appropriate control of S. iniae and S. parauberis disease. We suggested the phenotype observation along with universal primer combination to detect any kind of infection or carriers at early stages.
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