Stress Inducible Overexpression of Arabidopsis Nucleotide Diphosphate Kinase 2 Gene Confers Enhanced Tolerance to Salt Stress in Tall Fescue Plants
2017
Lee, K.W., National Institute of Animal Science, RDA, Chenoan, Republic of Korea | Kim, Y.G., Gyeongsang National University, Jinju, Republic of Korea | Md. Atikur Rahman, National Institute of Animal Science, RDA, Chenoan, Republic of Korea | Kim, D.H., Gyeongsang National University, Jinju, Republic of Korea | Iftekhar Alam, National Institute of Biotechnology, Dhaka, Bangladesh | Lee, S.H., National Institute of Animal Science, RDA, Chenoan, Republic of Korea | Kim, Y.H., Gyeongsang National University, Jinju, Republic of Korea | Kwak, S.S., Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea | Yun, D.J., Konkuk University, Seoul, Republic of Korea | Lee, B.H., Gyeongsang National University, Jinju, Republic of Korea
Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) is an upstream signaling molecule that has been shown to induce stress tolerance in plants. In this study, the AtNDPK2 gene, under the control of a stress-inducible SWPA2 promoter, was introduced into the genome of tall fescue (Festuca arundinacea Schreb.) plants. The induction of the transgene expression mediated by methyl viologen (MV) and NaCl treatments were confirmed by RT-PCR and northern blot analysis, respectively. Under salt stress treatment, the transgenic tall fescue plants (SN) exhibited lower level of H₂O₂ and lipid peroxidation accumulations than the non-transgenic (NT) plants. The transgenic tall fescue plants also showed higher level of NDPK enzyme activity compared to NT plants. The SN plants were survived at 300 mM NaCl treatment, whereas the NT plants were severely affected. These results indicate that stress-inducible overexpression of AtNDPK2 might efficiently confer the salt stress tolerance in tall fescue plants.
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