Optimization of Extraction Conditions and Investigation of Stability of Solanum nigrum Extract with Anti-Yeast Activity
2017
Seo, Y.S., Jeonnam Bioindustry Foundation, Jangseong, Republic of Korea | Yang, E.J., Jeonnam Bioindustry Foundation, Jangseong, Republic of Korea
Hot water extracts of 24 medicinal plants were screened for their anti-yeast activities against Zygosaccharomyces rouxii. Solanum nigrum extract showed only anti-yeast activity. To develop a natural anti-yeast preservative from S. nigrum, extraction yield and anti-yeast activity of S. nigrum extract were investigated using various extraction solvents, solid-solvent ratios, temperatures, and times. The results showed that the optimum extraction conditions for anti-yeast S. nigrum were as follows: 50% ethanol, solid-solvent ratio of 1:25, extraction temperature of 70℃, and extraction time of 4 h. The antimicrobial activity of S. nigrum extract was tested against food-spoilage bacteria, yeasts, and molds. S. nigrum extract exhibited strong antifungal activity with minimum inhibitory concentrations of 39~625 μg/mL against five yeasts and three molds. The stability of anti-yeast activity of S. nigrum extract was examined under different conditions of temperature, pH, and NaCl concentration. The anti-yeast activity of S. nigrum extract was stable at -20∼121℃, pH 3~9, and 0~10% NaCl. However, the anti-yeast activity of S. nigrum extract was reduced by 2-fold under 15% and 20% NaCl conditions.
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