Functional Analysis of the HD-Zip I Gene ZmHDZ1 in ABA-mediated Salt Tolerance in Rice
2017
Qianqian Wang, Anhui Agricultural University, Hefei, China | Kangyong Zha, Anhui Agricultural University, Hefei, China | Wenbo Chai, Anhui Agricultural University, Hefei, China | Yu Wang, Anhui Agricultural University, Hefei, China | Bin Liu, Anhui Agricultural University, Hefei, China | Haiyang Jiang, Anhui Agricultural University, Hefei, China | Beijiu Cheng, Anhui Agricultural University, Hefei, China | Yang Zhao, Anhui Agricultural University, Hefei, China
The homeodomain-leucine zipper I (HD-Zip I) transcription factors play crucial roles in the regulation of abiotic stress responses, whereas, up to now, most of them are still functionally unknown. In present study, we identified a maize HD-Zip I gene, designing as ZmHDZ1, and investigated its potential roles in salt stress response. The qRT-PCR results showed that expression of ZmHDZ1 was induced by ABA and salt stress. Transactivation assay and transient expression analyses indicated that ZmHDZ1 protein was located in the nucleus in tobacco leaf cells and had the transactivation activity in yeast. Over-expression of ZmHDZ1 in rice reduced tolerance to salt stress, thus, led to greater accumulation of malondialdehyde (MDA) as well as higher level of relative electrolyte leakage (REL) compared to WT plants. In addition, the transgenic seedlings also increased sensitivity to exogenous ABA. Furthermore, the expression levels of four salt responsive genes were different between transgenic and WT rice under normal or NaCl treatment. These results suggested that ZmHDZ1 function as a negative regulator in response to salt stress through the ABAmediated signal transduction pathways.
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