Overexpression of the Receptor-Like Cytoplasmic Kinase Gene XCRK Enhances Xoc and Oxidative Stress Tolerance in Rice
2017
Yuxia Zhang, Xiamen University, Xiamen, China | Xiaoling Guo, Xiamen University, Xiamen, China | Yuchao Cui, Xiamen University, Xiamen, China | Chiming Guo, Xiamen University, Xiamen, China | Liang Chen, Xiamen University, Xiamen, China
In this paper, we characterized a differentially expressed receptor-like cytoplasmic kinase XCRK, which confers resistance to bacterial leaf streak (BLS). We analyzed the tissue expression of XCRK and showed that XCRK was widely expressed in multiple rice (Oryza sativa) organs, including internodes, roots, leaves and flowers. In addition, the expression of XCRK was significantly induced by ABA, salt and H₂O₂ treatments, suggesting its function in these pathways. The XCRK-overexpressing transgenic seedlings exhibited higher tolerance to Xanthomonas oryzae pv.oryzicola (Xoc) compared with the wild-type seedlings. Furthermore, XCRK-overexpressing seedlings showed stronger antioxidant capacity with reduced MDA and H₂O₂ content and higher antioxidant enzyme activities. It has been hypothesized that the enhanced Xoc tolerance was attributed to the improved expression of resistance-responsive factors positively regulated by XCRK. In accordance with this, the expression of resistance and oxidation-related genes Wrky77, Wrky13, PAL1, PR5, Fe-SOD and SodCc2 were up-regulated by the overexpression of XCRK, which might contribute collectively to the increased Xoc tolerance. Overall, overexpression of XCRK could enhance the antioxidant capacity and Xoc tolerance in rice.
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