A novel M2e-multiple antigenic peptide providing heterologous protection in mice
2016
Wen, F., Chinese Academy of Agricultural Sciences, Shanghai, China | Ma, J.H., Chinese Academy of Agricultural Sciences, Shanghai, China | Yang, F.R., Chinese Academy of Agricultural Sciences, Shanghai, China | Huang, M., Chinese Academy of Agricultural Sciences, Shanghai, China | Zhou, Y.J., Chinese Academy of Agricultural Sciences, Shanghai, China | Li, Z.J., Chinese Academy of Agricultural Sciences, Shanghai, China | Wang, X.H., Chinese Academy of Agricultural Sciences, Shanghai, China | Li, G.X., Chinese Academy of Agricultural Sciences, Shanghai, China | Jiang, Y.F., Chinese Academy of Agricultural Sciences, Shanghai, China | Tong, W., Chinese Academy of Agricultural Sciences, Shanghai, China | Tong, G.Z., Chinese Academy of Agricultural Sciences, Shanghai, China
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
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