Heterometal-Coordinated Monomeric Concanavalin A at pH 7.5 from Canavalia ensiformis
2017
Chung, N.J., Chonbuk National University, Jeonju, Republic of Korea | Park, Y.R., Chonbuk National University, Jeonju, Republic of Korea | Lee, D.H., Chonbuk National University, Jeonju, Republic of Korea | Oh, S.Y., Chonbuk National University Medical School, Jeonju, Republic of Korea | Park, J.H., Chonbuk National University, Iksan, Republic of Korea | Lee, S.J., Chonbuk National University, Jeonju, Republic of Korea
The structure of concanavalin A (ConA) has been studied intensively owing to its specific interactions with carbohydrates and its heterometal (Ca2+ and Mn2+) coordination. Most structures from X-ray crystallography have shown ConA as a dimer or tetramer, because the complex formation requires specific crystallization conditions. Here, we reported the monomeric structure of ConA with a resolution of 1.6 A, which revealed that metal coordination could trigger sugar-binding ability. The calcium coordination residue, Asn14, changed the orientation of carbohydrate-binding residues and biophysical details, including structural information, providing valuable clues for the development and application of detection kits using ConA.
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