Immune Enhancement Effect of Asterias amurensis Fatty Acids through NF-κB and MAPK Pathways on RAW 264.7 Cells
2018
Chaiwat Monmai, Gangneung-Wonju National University, Gangneung, Republic of Korea | Go, S.H., Gangneung-Wonju National University, Gangneung, Republic of Korea | Shin, I.S., Gangneung-Wonju National University, Gangneung, Republic of Korea | You, S.G., Gangneung-Wonju National University, Gangneung, Republic of Korea | Lee, H., Dankook University, Cheonan, Republic of Korea | Kang, S.B., National Institute of Horticultural and Herbal Science, RDA, Seogwipo, Republic of Korea | Park, W.J., Gangneung-Wonju National University, Gangneung, Republic of Korea
Asterias amurensis is a marine organism that causes damage to the fishing industry worldwide; however, it has been considered a promising source of functional components. The present study aimed to investigate the immune-enhancing effects of fatty acids from three organs of A. amurensis on murine macrophages (RAW 264.7 cells). A. amurensis fatty acids boosted production of immune-associated factors such as nitric oxide (NO) and prostaglandin E2 in RAW 264.7 cells. A. amurensis fatty acids also enhanced the expression of critical immuneassociated genes, including iNOS, TNF-α, IL-1β, and IL-6, as well as COX-2. Western blotting showed that A. amurensis fatty acids stimulated the NF-κB and MAPK pathways by phosphorylation of NF-κB p-65, p38, ERK1/2, and JNK. A. amurensis fatty acids from different tissues resulted in different levels of NF-κB and MAPK phosphorylation in RAW 264.7 cells. The results increase our understanding of how A. amurensis fatty acids boost immunity in a physiological system, as a potential functional material.
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