QTLs associated with reproductive stage salinity tolerance derived from NSIC RC222 x Jambo Jet BC1F2 mapping population
2017
Ordonio, J.L. | Vergara, G.V. | Diaz, Ma.G.Q. | Gregorio, G.B. | Hernandez, J.E.
Ten BC1F2 families derived from the cross between NSIC RC222 (salt-sensitive) and Jumbo Jet (salt tolerant) were screened for reproductive stage salinity tolerance (EC=6 dS raised to negative 1). Based on segregation pattern, T109290 (BC1F2-JJ29-P1) was selected and used as mapping population wherein 184 lines from this family grown under normal condition were randomly selected and genotyped. The genotypic data was used to correlate with the morphological traits of the 184 BC1F3-JJ29-P1 lines evaluated at reproductive stage under salinized condition. Exactly 194 markers were used to construct the genetic linkage map using inclusive composite interval mapping (ICIM) software version 4.1. The linkage map has a total length of 397.45 cm with an average interval size of 2.05 cm. Nine QTLs were identified at reproductive stage LOD 3.0 located on chromosomes 1, 4, 6, 7 and 11 explaining 2.69 to 70.06% of the phenotypic variance. These include QTLs for salinity evaluation score (qSES 1.1), days to flowering (qDFL 6, gDFL 11.1, qDFL 11.2), plant height (qPTH 1, qPTH 4), number of fertile spikelets (qNFS 7) and number of sterile spikelets (qNSS 1, qNSS 4). It is recommended that fine-mapping of the identified QTLs be done to help identify closely linked markers for use in marker-assisted backcrossing.
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