Screening of potato germplasm against potato leaf roll virus and its management through resistance inducer chemicals

Potato tuber (Solanum tuberosum L.) is fourth largest staple food in world followed by wheat, rice and maize. In Pakistan, Potato leaf roll disease is one of the most important diseases of potato. It is transmitted by green peach aphid (Myzus persicae) and causes 90% losses. Screening trial of twenty varieties/lines of potato was established against PLRV. For the management of disease seven plant activators at (1mM, 2mM, 3mM) concentration were used for inducing systemic acquired resistance. As an aim of this study, efficient and environment friendly management of this disease is envisaged by activating the systemic resistance of potato plants against the disease by the application of resistance inducing chemicals. The experiment was conducted in the research area of Plant Virology Section, Ayub Agricultural Research Institute, Faisalabad. Randomized complete block design (RCBD) was used for field trials with three replication. Disease incidence was analyzed statistically by Least Significance difference. Among twenty varieties/lines, ten varieties (SL 15-10, FD 63-1, FD 78-36, Sante, FD 74-21, SL 5-2, FD 76-18, FD 61-3, SL 15-10, SL 14-15 ) were found resistant, five varieties (Simply Red, FD 35-36, FD 73-73, FD 78-51, SL 13-43) were moderately resistant, one (FD 76-67) was moderately Susceptible, two (FD 71-1, SL 9-14) were moderately susceptible and two (FD 77-4, Cardinal) were highly suscepti12]e. Serological test (DAS-ELlSA) was performed for the detection of PLRV and detects the virus in varieties such as Three varieties (SL 15-10, FD 63-1, Sante) were found resistant, eleven varieties (SL 5-2, FD 76-18, FD 61-3, SL 15-10, SL 14-15, FD 78-36, Simply Red, FD 35-36. FD 73-73, FD 78-51, SL 13-43) were moderately resistant, two varieties (FD 76-67, FD 78-51) were Moderately Susceptible, two (FD 71-1, SL 9-14) were moderately susceptible and four varieties (FD 71-1, FD 77-4, SL 9-14, Cardinal) were susceptible. Among six inducer chemicals (Ascorbic acid, Salicylic acid, Benzoic acid, Oxalic acid, Zinc sulphate and Citric acid). The concentration 3mM of Salicylic acid expressed maximum disease reduction (51.85%) as compared to control. While Oxalic acid at 1mM concentration showed minimum disease reduction (26.29%) as compared to control.