Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV. 42 virusa Newcastle bolesti živine / Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus
2015
Milić, Nenad (Fakultet veterinarske medicine, Beograd (Srbija)) | Nišavić, Jakov (Fakultet veterinarske medicine, Beograd (Srbija)) | Borozan, Sunčica (Fakultet veterinarske medicine, Beograd (Srbija)) | Zorić, Andrea (Fakultet veterinarske medicine, Beograd (Srbija)) | Lazić, Sava (Naučni institut za veterinarstvo Novi Sad, Novi Sad (Srbija)) | Petrović, Tamaš (Naučni institut za veterinarstvo Novi Sad, Novi Sad (Srbija)) | Rašić, Zoran (Veterinarski specijalistički institut Jagodina, Jagodina (Srbija))
The objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra- SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines.
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