Uticaj 2,3,5-trijodbenzoeve kiseline i jona srebra na ožiljavanje Robinia sp. - in vitro / Influence of 2,3,5-triiodobenzoic acid and silver ions on rooting in Robinia sp.: In vitro
2013
Kovačević, Branislav (Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad (Srbija)) | Orlović, Saša (Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad (Srbija)) | Katanić, Marina (Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad (Srbija)) | Vasić, Sreten (Institut za nizijsko šumarstvo i životnu sredinu, Novi Sad (Srbija))
Beside well known utilization of black locust (Robinia pseudoacacia L.) in biomass production and bee-keeping, there are numerous Robinia species and genotypes of ornamental value. They are of particular interest to be propagated by means of tissue culture, where the shoot rooting is a significant phase. Results of the research of the influences of 2,3,5-triiodobenzoic acid (TIBA), well known inhibitor of indolacetic acid (IAA) polar transport and silver ions (Ag+ ), known to suppress the activity of ethylene were examined in this work. Two genotypes were examined: Robinia pseudoacacia L. cl. RP, the genotype of good rooting ability on medium without gorwth regulators in vitro and R. pseudoacacia x R. luxurians cl. Rózsaszín AC, whose rooting is poor on such a medium. Results gained after six weeks of cultivation suggest the significant effect of the concentration of TIBA on number of roots and significant effect of the interaction genotype x c(TIBA) on number of roots and percentage of rooted shoots. The presence of silver ions effected significantly number of roots per shoot through the interaction genotype x c(Ag+ ). The signifficance of gained results for further work on rooting Robinia shoots in vitro was disccused.
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