Application of ATR-FTIR analysis for determination of fumonisins in corn
Jakšić, Sandra M. (Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)) | Jajić, Igor M. (Faculty of Agriculture, Novi Sad (Serbia). Department of Animal Science) | Despotović, Vesna N. (Faculty of Science, Novi Sad (Serbia). Department of Biology and Ecology) | Živkov-Baloš, Milica M. (Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)) | Stojanov, Igor M. (Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)) | Krstović, Saša Z. (Faculty of Agriculture, Novi Sad (Serbia). Department of Animal Science) | Mašić, Zoran S. (Scientific Veterinary Institute Novi Sad, Novi Sad (Serbia)) | Abramović, Biljana F. (Faculty of Science, Novi Sad (Serbia). Department of Biology and Ecology)
In order to develop rapid, inexpensive and, at the same time, reliable method for the analysis of molds of the genus Fusarium as an indicator of the presence of fumonisins in corn samples, possible application of Fourier transform infrared spectroscopy (FTIR) with attenuated total reflection (ATR) technique was examined. The content of fu- monisins in contaminated corn samples had previously been quantified by ELISA method. At the spectrum of the sample contaminated with a high concentration of mycotoxins, there was a lack of the peak at 1,743 cm–1, but the peak was observed at 1709 cm–1. To the purpose of result classification the principal component analysis (PCA) and cluster analysis were applied. Conclusions of the two methods were similar both when applying ATR technique in the whole region of the spectrum (1,150–1,770 cm–1) and when the whole spectrum was di- vided into two regions: 1,150–1,450 and 1,450–1,770 cm–1. However, classification of samples was somewhat better in the ranges 1,150–1,770 and 1,450–1,770 cm–1. Of the 16 analyzed corn samples, only very contaminated corn sample with 190 mg/kg was correctly classified as compared to the other samples with the content of less than 10 mg/kg. Also, it was found that evaluation of fumonisins in corn by this technique requires further investigation encom- passing recording of spectra of contaminated corn of the same genotype in order to avoid the possible impact of different hybrids on the spectrum.
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