Direct method for regiospecific analysis of TAG using [alpha]-MAG
Turon, Fabrice | Bachain, Philippe | Caro, Yanis | Pina, Michel | Graille, Jean
An analytical procedure was developed for regiodistribution analysis of TAG using [alpha]-MAG prepared by an ethyl magnesium bromide deacylation. In the present communication, the deacylation procedure is shown to lead to representative [alpha]-MAG, allowing the composition of the native TAG in the a-position to be determined directly. The composition in the [bêta]-position can then be estimated from the composition of the [alpha]-MAG and TAG according to the formula 3 x TAG - 2 x [alpha]-MAG. The estimates are superior to those obtained using the [alpha],[bêta]-DAG and Brockerhoff calculations as they come closer to the theoretical value and have smaller SID. The present procedure, first demonstrated on a synthetic TAG, was then successfully applied to the analysis of borage oil, milkfat, and tuna oil. (Résumé d'auteur)
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