Characterization of Pyrenophora avenae Isolated from Discolored Black Oat Seeds in Korea
2018
Choi, J.H., National Institute of Agricultural Sciences, Wanju, Republic of Korea | Kim, J., National Institute of Agricultural Sciences, Wanju, Republic of Korea | Ham, H., National Institute of Agricultural Sciences, Wanju, Republic of Korea | Lee, T., National Institute of Agricultural Sciences, Wanju, Republic of Korea | Nah, J.Y., National Institute of Agricultural Sciences, Wanju, Republic of Korea | Choi, H.W., National Institute of Agricultural Sciences, Wanju, Republic of Korea | Lee, Y.K., National Institute of Agricultural Sciences, Wanju, Republic of Korea | Hong, S.K., National Institute of Agricultural Sciences, Wanju, Republic of Korea
In January 2017, discolored black oat seeds were found in the storage depot of a farmhouse in Jeongeup. Pyrenophora sp. was detected in 45% of the oat seeds surveyed. All Pyrenophora isolates obtained from the seeds were identified as Pyrenophora avenae based on the sequences of internal transcribed spacer (ITS) rDNA regions and glyceraldehyde 3-phosphate dehydrogenase (GPDH) gene and validated by morphological and cultural characterization. A phylogenetic tree constructed using the ITS and GPDH sequences showed that the Korean isolates of P. avenae comprise of four genetically distinct groups. Pathogenicity test validated that the fungus is an infectious agent responsible for discolored black seeds and leaf blotch in oat plants. This is the first study report that P. avenae causes leaf blotch disease of oat in Korea.
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