Rapid monitoring of proline accumulation in paprika leaf sap relative to leaf position and water stress
Shiva Ram Bhandari, Chonbuk National University, Jeonju, Republic of Korea | Lee, M.J., Chonbuk National University, Jeonju, Republic of Korea | Rhee, H.C., National Institute of Horticulture and Herbal Science, Rural Development Administration, Haman, Republic of Korea | Choi, G.L., National Institute of Horticulture and Herbal Science, Rural Development Administration, Haman, Republic of Korea | Oh, S.S., Gyeongnam Agricultural Research and Extension Services, Jinju, Republic of Korea | Lee, J.T., Fruit Vegetable Research Institute, Jeonbuk ARES, Gunsan, Republic of Korea | Lee, J.G., Chonbuk National University, Jeonju, Republic of Korea
The present study evaluated the pattern of proline accumulation in paprika leaf sap using a modifed high-performance liquid chromatography (HPLC) method. Leaf sap samples were extracted using fve diferent solvents and two extraction procedures, and were analyzed using modifed HPLC analytical conditions. Enhanced extraction and HPLC analytical conditions were applied to analyze paprika leaf sap obtained from diferent plant parts (petiole and lamina sap) under diferent environmental conditions. The results showed that pure water extraction without heating or organic solvent performed best. This newly developed HPLC method was validated and confrmed suitable for analyzing proline content in leaf sap. The proline concentration in both the petiole and lamina sap showed a similar accumulation pattern, exhibiting the lowest proline concentration in the arbitrary node group I (1-5 nodes) and the highest concentration in the VI node group (21-30 nodes). In addition, sap from both the petiole and lamina of side stem leaves showed statistically higher proline content than the main stem leaves. The proline concentration also increased with increasing water stress during both the fruit enlargement and coloring stages, but primarily during the fruit coloring stage. The overall results suggest that the proline concentration in paprika leaves diferentially accumulates throughout the plant and can be analyzed by water extraction using a modifed HPLC method.
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