Improved characterization of Clematis based on new chloroplast microsatellite markers and nuclear ITS sequences
Zhigao Liu, Zhejiang A and F University, Lin'an, China | Helena Korpelainen, University of Helsinki, Helsinki, Finland
Currently, there is a lack of genetic markers capable of efectively detecting polymorphisms in Clematis. Therefore, we developed new markers to investigate inter- and intraspecifc diversity in Clematis. Based on the complete chloroplast genome of Clematis ternifora, simple sequence repeats were explored and primer pairs were designed for all ten adequate repeat regions (cpSSRs), which were tested in 43 individuals of 11 Clematis species. In addition, the nuclear ITS region was sequenced in 11 Clematis species. Seven cpSSR loci were found to be polymorphic in the genus and serve as markers that can distinguish diferent species and be used in diferent genetic analyses, including cultivar identifcation to assist the breeding of new ornamental cultivars.
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