Natural occurrences and host range studies of Cucumber mosaic virus (CVM) infecting ornamental species in the Rawalpindi-Islamabad area of Pakistan
2017
Ashfaq, M. | Saleem, A. | Waqas, M. | Mukhtar, T.
Surveys were conducted during the spring and autumn of 2012 and 2013 to determine the presence, prevalence and distribution of Cucumber mosaic virus (CVM) on ornamental plants in the Rawalpindi-Islamabad area of Pakistan. A total of 1,783 symptomatic leaf samples (1,733 from ornamentals and 50 from weeds) were collected from 79 ornamental plant species in propagation sites such as nurseries, private gardens and public areas including parks and street-grown ornamentals. All the samples were subjected to double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using monoclonal antibodies for the detection of CVM. The disease incidence varied from season to season and nursery to nursery. The highest incidence of 46.47% and 35.01% of CVM was observed in public areas and propagation sites, respectively, during autumn 2012. Similarly, the incidence was 59.28% and 47.36% during autumn 2013 And 66.5% and 51.69% during spring 2013 in public areas and propagation sites, respectively. Of the 79 ornamental plant species inoculated with gerbera isolate of CVM, 36 species were ELISA positive while the rest showed negative response. Among the tested weed flora of ornamentals, Oxalis spp., Ixora spp., Cyperus rotundus and Portulaca somnifera were also found to be infected with CVM.
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