Monitoring of neonicotinoid residues in honey samples from autonomous province of Vojvodina
2016
Jovanov, Pavle | Sakač, Marijana | Kos, Jovana | Šarić, Bojana | Ivezić, Aleksandar | Nedeljković, Nataša | Pojić, Milica
After the European Union and Republic of Serbia temporary banned the use of the neonicotinoids in flowering crops that honeybee might visit, there has been an increased interest on their residues determination, especially in honeybee products such as honey. Neonicotinoids are systemic insecticides translocated to the whole plant (flowers, pollen and nectar). This group of insecticides includes nitro-subsituted (dinotefuran, nitenpyram, thiamethoxam, imidacloprid and clothianidin) and cyano-substituited (acetamiprid and thiacloprid) compounds. The objective of this study was to conduct the monitoring of neonicotinoids residues in collected honey samples from Autonomous Province of Vojvodina using developed HPLC-MS/MS analytical method with QuEChERS sample preparation procedure for the analysis. The liquid chromatographic conditions were optimized by Response surface methodology with Box–Behnken design and the global Derringer´s desirability. The chosen method was fulfilling the of SANCO/12571/2013 validation requirements. Used methodology provided all necessary requirements for a good fitted analytical method, such as good accuracy (R, 70–120%), repeatability (RSD, < 20%) and within-laboratory reproducibility (RSD, <20%), limit of detection (LOD, 0.5–1.0 µg kg-1) and quantification (LOQ, 1.5–2.5 µg kg-1). Matrix effects were compensated by the use of matrix-matched calibration. For the second time, monitoring of neoniccotinoids residues included more than 120 honey samples of different plant origin (sunflower, linden, acacia, and wildflower) collected from more than 50 locations in all 7 counties of Autonomous Province of Vojvodina. Residue analysis discovered the presence of thiamethoxam, clothianidin and imidacloprid in detectable concentrations bellow MRLs, therefore implicating the necessity and importance of ongoing control of this type of food.
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