Selection of industrially valuable xylanase producer strain
2015
Xylanase (hemicellulase) is one of the key enzymes in the creation industrial process of natural biopolymers enzymatic degradation. The importance of this enzyme from the viewpoint of alternative sugar and biofuel production from plant row materials is attracting more and more attention. The objective of the present study was the primary selection of xylanase producing fungi from soil - rich microflora sources of the Caucasus, selection and optimization of the carbon content and nitrogen components in nutrient media under submerged fermentation for industrial xylanase production. Initially, fungal strains, including extremophiles, were isolated from different soils of climatic zones of Georgia. In total, more than 420 strains were isolated and purified. Among them 85 were defined as extremophilic strains (halophiles). Finally, 21 most active producers of xylanase were chosen from this collection. Further selection revealed five strains representing the following genera: Aspergillus, Penicillium, Sporotrichum and Trichoderma according to their relatively high activity of extracellular xylanase (with maximum 52 U/ml, Penicillium canescence AEM 85). Nutrient medium for xylanase cultivation in submerged condition for P. canescene AEM 85 was optimized. The xylanase activity selected producer, while the growth on the optimized medium as compared with the basic medium, increased by more than 40%.
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