Methodology for studying arbuscular-mycorrhizal fungi of wheat rhizosphere.
2019
Mycorrhizal fungi are the main component of the soil micro biota in most of the agro-ecosystems and colonize nearly 90% of plant species roots. They are generally known to increase the absorption and translocation of mineral nutrients from the soil to the host plants to improve the tolerance of the host plant towards biotic and abiotic stress factors. Research about mycotrophy of the plants has been started since the second half of the 20th century in Georgia. Georgian scientists described colonization of plant roots with Arbuscular mycorrhiza fungi but it has to be mentioned that AM fungal biodiversity are less likely to be studied as there was no attempt to isolate AMF spores from the soil. The aim of the article was to elaborate a simple method of isolation and visualization of AMF spores from the wheat rhizosphere. The method made it possible to visualize and document photographically extra radical hypha, chlamydospores, intracellular mycelium and vesicles from wheat rhizosphere and roots. Fig. 2, Ref. 17.
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