RNAi for Plant Functional Genomics
2004
Matthew, Louisa(CSIRO Plant Industry, GPO Box 1600)
A major challenge in the post-genome era of plant biology is to determine thefunctions of all the genes in the plant genome. A straightforward approach to thisproblem is to reduce or knock out expression of a gene with the hope of seeing aphenotype that is suggestive of its function. Insertional mutagenesis is a useful toolfor this type of study, but it is limited by gene redundancy, lethal knock-outs, nontaggedmutants and the inability to target the inserted element to a specific gene. RNAinterference (RNAi) of plant genes, using constructs encoding self-complementary‘hairpin’ RNA, largely overcomes these problems. RNAi has been used very effectivelyin Caenorhabditis elegans functional genomics, and resources are currently beingdeveloped for the application of RNAi to high-throughput plant functional genomics.
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