Optimization of Deodeok (Codonopsis lanceolata) Extraction Condition Using Response Surface Methodology
2019
Kim, Y.S., Daegu University, Gyeongsan, Republic of Korea | Kim, S.H., Daegu University, Gyeongsan, Republic of Korea
The deodeok extraction conditions were optimized by a response surface methodology (RSM). The physicochemical properties and major components of the deodeok extracts were analyzed. A central composite design was applied to examine the effects of three independent variables: solvent to sample ratio (10~30 mL/g; X1), extraction temperature (70~90¡É; X2), and extraction time (1~5 hour; X3). The extraction conditions were optimized with the characteristics of the extracts as a dependent variable. As a result, the extraction yields were 35.01~71.53% and the coefficient (R©÷ ) of the derived equation from the response surface regression for extraction yield was 0.9574. The total sugar and reducing sugar contents in all extracts were 3.19~6.04 mg/mL and 1.58~3.11 mg/mL, respectively, which were within the 1% significance level. The crude saponin contents in all extraction samples were 0.09~0.17% with a R©÷ value of 0.9373 and were within the 1% significance level. The lobetyolin content as a major component in all extraction samples was 16.72~32.82 ¥ìg/mL with a R©÷ value of 0.9791 that was within the 1% significance level. The optimal conditions based on the superimposing of the four-dimensional RSM on the crude saponin and lobetyolin contents under various conditions were established as follows: solvent to sample ratio, 12 mL/g; extraction temperature, 75¡É; and extraction time, 3 hours.
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