Enhanced Production of Protopanaxatriol from Ginsenoside Re and Rg1 Using a Recombinant Bacterial β-glucosidase
Siddiqi, M.Z., Hankyong National University, Anseong, Republic of Korea | Jin, M.F., Hankyong National University, Anseong, Republic of Korea | Song, B.K., Dalian Polytechnic University, Dalian, China | Park, H.Y., National Institute of Biological Resources, Incheon, Republic of Korea | Oh, J.M., Chonbuk National University, Republic of Korea | Chun, S.K., Chonbuk National University, Republic of Korea | Jin, F.X., Dalian Polytechnic University, Dalian, China | Yu, H.S., Dalian Polytechnic University, Dalian, China | Im, W.T., Hankyong National University, Anseong, Republic of Korea
20(S)-protopanaxatriol (PPTA), an aglycone of ginseng saponins, is rarely found and is present in a limited amount; it exhibits various pharmaceutical activities, including memory improvement, and anti-diabetic and anti-cancer effects. An enzymatic method was developed to improve 20(S)-protopanaxatriol aglycone [20(S)-PPTA] production using recombinant β-glucosidase (BgpA) cloned from Terrabacter ginsenosidimutans Gsoil 3082∨T . The optimal hydrolytic activity was obtained at pH 7.0 and 37℃. The enzyme could hydrolyze the rutinoside and glucose moieties at the C6 and C20 positions, respectively, of ginsenosides Re and Rg1 to produce 20(S)-PPTA through the formation of ginsenoside F1 as an intermediate. For gram-scale production, the enzyme reaction was performed in a 10-L fermenter for 48 h at a substrate concentration of 10 mg/mL. Finally, 5.45 g 20(S)-PPTA was produced from 36 g PPT type ginsenoside mixture with 95% purity by chromatography. This study thus reveals an industrial application of a minor component, 20(S)-PPTA.
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