Systematic optimization of coding sequence for functional protein expression
2015
Oost, van der, J.
The present invention relates to a systematic approach to codon selection for provision of a coding sequence for functional expression of a heterologous protein in a host cell. This approach recognizes that non-optimal codons for translation efficiency may be desirable at certain sites and relies on the provision of an expression library of synonymous coding sequences for the protein of concern. Expression of each such sequence is compared under the same conditions in the host cell, desirably by high-throughput screening.
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Эту запись предоставил Wageningen University & Research