Agricultural waste after cultivation of Pleurotus ostreatus as a potential biomass resource for ethanol production
2018
Tummo, K. | Thammasittirong, A. | Thammasittirong, S.N-R.
Spent mushroom substrate (SMS) gathered after Pleurotus ostreatus cultivation was investigated as a potential biomass resource for ethanol production. The chemical composition, morphological characteristics, total saccharized sugars obtained from enzyme hydrolysis and ethanol production from SMS were compared with those from mushroom substrate (MS). The experiments were conducted at Kasetsart University, Khampaeng Saen Campus, Nakhon Pathom province, Thailand from 2016-2017. SMS had lower lignin content than MS, but higher cellulose and hemicellulose (58.15%). Analysis through scanning electron microscope (SEM) showed that the SMS had a large numbers of pores than MS, leading to an increased availability of surface area for pre-treatment and enzymatic activity. These results are related to findings indicating that SMS requires milder pre-treatment conditions and easier saccharification through enzymatic hydrolysis than MS. When SMS was used as a substrate for the simultaneous saccharification and fermentation (SSF) process, the maximum ethanol concentration produced from SMS hydrolysate by Saccharomyces cerevisiae ATKU132 was 10.00 g/L, while the corresponding value for MS hydrolysate was 4.12 g/L. These results indicated that SMS, available from agricultural waste all year round, can be used as a potential source of raw material for bioethanol production.
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