Ambient storage in mid-elevation environment maintains seed viability and seedling vigor of TGMS hybrid parentals
2018
Salazar, B.T. | Guittap, L.V. | Masajo, T.M.
Low-temperature environment is critical in seed production of TGMS female parent line. The study was conducted from Sept 2017 to March 2018 to determine if mid-elevation environment can also be effective in keeping seed viability and seedling vigor of TGMS hybrid parental lines. A 3 x 2 x 2 factorial experiment was laid out in split-split plot design with storage environment (ambient storage room, cold storage room, and mid-elevation storage room) as main plot, storage container (woven polypropylene bag, and woven polypropylene bag with polyethylene plastic as liner) as subplot, and hybrid TGMS parental line (M20 female parent and M20 male parent) as sub-subplot. Seed testing commenced before storage and every two weeks thereafter until 24 weeks after storage (WAS). Seed viability was assessed using filter paper method, while seedling vigor was evaluated using seedling length and biomass parameters. Comparable seed viability and seedling vigor were observed across treatments until 14 WAS. Germination percentage (GP) of seeds stored in the ambient room significantly declined to 84% at 14 WAS, and further to 63% at 24 WAS. Interestingly, mid-elevation storage registered high GP comparable to that in cold storage (greater than equal to 90%) until 16 WAS. Results also showed that, regardless of storage environment, woven polypropylene bag with plastic liner was 10% more effective in maintaining GP compared to container without liner. Storage treatments were also found to extend high GP up to 10 and 18 WAS in M20 female and M20 male parentals, respectively.
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