Biofortified indica rice attains iron and zinc nutrition dietary targets in the field
2016
enzo lombi | gerard barry | elad tako | kurniawan r. trijatmiko | alexander a. t. johnson | conrado dueñas | jessica rey | jeanette balindong | hiromi nakanishi | jaime borrero | cheryl adeva | felichi mae arines | joe tohme | maria v. sapasap | raymond p. glahn | james stangoulis | norman oliva | inez h. slamet-loedin | perigio francisco | nikolaos tsakirpaloglou | lina torrizo | andy nelson | prabhjit chadha-mohanty
More than two billion people are micronutrient deficient. Polished grains of popular rice varieties have concentration of approximately 2 ?g g?1 iron (Fe) and 16 ?g g?1 zinc (Zn). The HarvestPlus breeding programs for biofortified rice target 13 ?g g?1 Fe and 28 ?g g?1 Zn to reach approximately 30% of the estimated average requirement (EAR). Reports on engineering Fe content in rice have shown an increase up to 18 ?g g?1 in glasshouse settings; in contrast, under field conditions, 4 ?g g?1 was the highest reported concentration. Here, we report on selected transgenic events, field evaluated in two countries, showing 15 ?g g?1 Fe and 45.7 ?g g?1 Zn in polished grain. Rigorous selection was applied to 1,689 IR64 transgenic events for insert cleanliness and, trait and agronomic performances. Event NASFer-274 containing rice nicotianamine synthase (OsNAS2) and soybean ferritin (SferH-1) genes showed a single locus insertion without a yield penalty or altered grain quality. Endosperm Fe and Zn enrichment was visualized by X-ray fluorescence imaging. The Caco-2 cell assay indicated that Fe is bioavailable. No harmful heavy metals were detected in the grain. The trait remained stable in different genotype backgrounds
Показать больше [+] Меньше [-]Kurniawan R. Trijatmiko et al., 'Biofortified indica rice attains iron and zinc nutrition dietary targets in the field', Scientific Reports, vol. 6(1), p.19792, Springer Nature, 2016
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил GARDIAN