Sequence data characterization and development of DNA markers for sesame (Sesamum indicum L.)

Ayse INCE; Mehmet KARACA

Sequence data characterization and development of DNA markers for sesame (Sesamum indicum L.)

2019

Ayse INCE (AKDENİZ ÜNİVERSİTESİ) | Mehmet KARACA (AKDENİZ ÜNİVERSİTESİ)

NewDNA sequencing techniques enable researchers obtain large quantity of sequenceinformation, which are deposited in digital storage or could be further minedfor other purposes. Sesame (Sesamumindicum L.) is one of very important oilseed crops, its seed oil containsmany antioxidant properties making sesame the queen of oil crops. Today,molecular plant breeding technology is indispensable for plant breeders and seedproducers. Compared to other seed oil crops the available number ofmicrosatellite markers in sesame is still not sufficient enough for thedevelopment of polymorphic markers for breeding and genetic studies. Thus, newapproaches or resources are needed for development of microsatellite markersfor sesame. In the present study, we utilized a total of 45099 transcribed genomicDNA sequences/expressed sequence tags and mined these sequences for studying frequencyof microsatellite motifs, ranging from di- to hexa-nucleotides with four to tentandem repeats, and repeat numbers greater than 10. Using mined transcribeddata, 42 putative microsatellite markers were developed and characterized atthe sequence level. However, we did not confirm these markers and have noinformation about the level of their polymorphisms in sesame in vitro. We discussed the biologicalmeaning of the motif lengths and repeat numbers in the sesame genome.

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