Optimisation of Panax ginseng liquid cell cultures for biomass accumulation and ginsenoside production.
2005
Kevers, Claire | Bare, Gislain | Gaspar, Thomas | Thonart, Philippe | Dommes, Jacques | HVOSLEF-EIDE, Anne Kathrine | PREIL, Walter
Solid calli and derived liquid cell cultures were initiated from one-year-old roots of Panax ginseng CA Meyer. Half-strength Murashige and Skoog medium supplemented classically with an auxin and a cytokinin did not appear favourable for biomass accumulation nor for a high ginsenoside content. Changes in the levels of mineral nutrients, sucrose and growth regulators were preliminary investigated here to improve growth and ginsenoside production in liquid cultures. The hypothesis that ginseng cells released growth inhibitors in the medium was not supported by the results obtained in experiments involving frequent transfers to fresh growth medium
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