Mcmaster method of worm egg count from faecal samples of goats: a comparison of single and double chamber enumeration of worm eggs

Chandrawathani P.; Premaalatha B.; Jamnah O.; Priscilla F. X.; Erwanas A. I.; Lily Rozita M. H.; Jackie P.; Josephin S. J. A. L.

Mcmaster method of worm egg count from faecal samples of goats: a comparison of single and double chamber enumeration of worm eggs

2015

Many parasitology laboratories practiced the McMaster technique as a method in obtaining the quantitative diagnosis of Strongyle eggs burden in farm animals especially ruminants. The McMaster technique also play a crucial role in faecal egg count reduction test (FECRT) for anthelmintic resistance identification. Some laboratoriesrecommend two-chamber counting method while some recommend single chamber counting method. This study focuses on the comparison between single and double counting in McMaster technique fordetection of Strongyle egg count. In this study, it is shown that there is no significant difference between both methods basedon the p-value obtained which is p>0.05 from 127 fresh goat faecal samples. The techniques practised during the study follow the standard established technique. Single chamber counting is suitable for a large number of faecal samples from big herds because it is faster, less laborious and produces sensitive and reliable results in Strongyle egg count. As more commercial farms are set up, there is a need to conduct a fast and efficient test to help farmers evaluate their livestock worm burden.

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