Protein extracted from Clitoria ternatea modulates genes related to diabetes in vivo
2020
Suwanto, A. | Gunawan, A. G. | Yanti | Minelko, M. | Ali, S.
Butterfly pea (Clitoria ternatea) is an indigenous plant from Moluccas in Indonesia. Due to its pharmacological properties, the flower of this plant is also used as a medicine in Ayurvedic practices in India. The present work was aimed to determine the antidiabetic activity of C. ternatea protein extract (PCT) via the inhibition of α-amylase activity in vitro, and the modulation of genes related to diabetes in diabetic mice in vivo. C. ternatea flower protein extract was prepared using isoprecipitation, and its efficacy as an antidiabetic candidate was tested in vitro through α-amylase inhibition assay, and in vivo through the measurement of blood glucose level and diabetes-related genes expression, including those that encode for peroxisome proliferator-activated receptors gamma (PPARγ), glucose transporter 2 (Glut2), transcription factor 7-like 2 (Tcf7l2), calpain-10 (Capn10), and monocyte chemoattractant protein 1 (MCP1) in alloxan-induced diabetic mice. PCT showed α-amylase inhibition in a dose-dependent manner. The highest α-amylase inhibition (20.63%) was observed at 1 mg/mL of PCT. The in vivo study showed that PCT decreased the blood glucose level in alloxan-induced diabetic mice. PCT also altered the expression of diabetes-related genes. In adipose and skeletal muscle tissues, PCT upregulated the gene expression of PPARγ and Tcf7l2, meanwhile MCP1 expression was downregulated. In the pancreas, PCT upregulated the gene expression of Glut2, Capn10, and Tcf7l2. These results indicate that PCT is a potential antidiabetic agent for the treatment of type 2 diabetes.
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