Vascular pathology associated with Leptospira icterohaemorrhagiae serovar lai strain Langkawi infection in guinea pigs (Cavia porcellus)
2018
Jasni S. | A. R. Bahaman | Fuzina N. H. | Tengku-Azmi T. I. | Tyagita, G. H.
Fifteen (15) guinea pigs were experimentally infected with Leptospira icterohemorrhagiae serovar Lai strainLangkawi, a new strain that was isolated from a human leptospirosis patient. Hematoxylin and Eosin ((H&E) stainingshowed haemorrhages, congestion and oedema in all internal organs examined (lungs, liver, spleen and kidneys) withinflammatory cell infiltration characterized by neutrophils, lymphocytes and macrophages. Hydropic degeneration andcell necrosis were also common in our findings. Leptospires were detected starting Day 2 p.i by silver staining andTransmission Electron Microscopy (TEM). Rise in antibody titres started on Day 5 p.i and leptospiral DNA wasdetected beginning Day 3 in the kidneys and Day 5 in the liver by Polymerase Chain Reaction (PCR) assay. Thefindings illustrated the pathogenesis of leptospirosis in guinea pigs which disclosed them as a suitable animal model fordemonstration of clinical symptoms of leptospirosis and pathological changes after being infected with Leptospiraicterohaemorrhagiae serovar Lai strain Langkawi, particularly pulmonary haemorrhages, a leading cause of mortalityin human leptospirosis.
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