Statistical optimisation of radial growth rates of Ganoderma neo-japonicum (KLUM61076) in low cost solid agar plate’s medium using full factorial design and central composite design
2015
Noorlidah Abdullah | Nik Hafizah Nik Ubaidillah | Muhammad Suffian Muhammad Annuar | Vikineswary Sabaratnam
The combination of low cost medium sources (brown sugar, spent yeast, molasses and corn steep liquor) for growth of Ganoderma neo-japonicum (KLUM61076) in solid agar plates was studied. The mathematical model of Full Factorial Design and Response Surface Methodology were used to select the optimum combination of carbon/nitrogen sources for the cultivation of G. neo-japonicum. The combination medium of brown sugar and spent yeast was the best for growth of G. neo-japonicum at 5.74% (w/v) carbon content in brown sugar and 0.06% carbon content (w/v) in spent yeast. The combination of two constituents contributed to a low requirement of C/N ratio 1.74 and gave the fastest mycelial growth rates (20.74 mm/day). This combinations of spent yeast/brown sugars showed an increment in mycelia yield, which is up to two-fold as compared with the standard commercial medium (yeast extract-glucose). The low cost medium has the potential to contribute to cost-effective cultivation for G. neo-japonicum large-scale production.
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