Hev b 1, Hev b 2 and Hev b 3 contents in natural rubber latex and powdered latex gloves

Siti Arija M. A.; Lau, C. H.; Chan, J. L.; Samsidar H.; Yeang, H. Y.; Hamilton, R. G.; Loke, Y. H.

Hev b 1, Hev b 2 and Hev b 3 contents in natural rubber latex and powdered latex gloves

2002

The contents of three latex allergens, Hev b 1, Hev b 2 and Hev b 3 were compared in 24 latex examination gloves and in fresh natural rubber latex using immunoassays that employed a two-site ELISA format. Hev b 1, Hev b 2 and Hev b 3 that were extractable from the powdered latex gloves were found to represent only a very small proportion of their contents in fresh latex and a very small proportion of the total extractable glove proteins. Of the three allergens studied, only Hev b 2 content was significantly correlated with total protein content. Hence, total protein content did not always reflect the contents of the individual latex allergens. Of the three allergens studied, Hev b 2 and Hev b 3 contents were significantly correlated with binding to IgE pooled from latex-allergic patients. One brand of glove had undetectable levels of the three individual latex allergens, indicating that it was feasible to remove a large proportion of the allergens during glove + manufacture.

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