Identification of fucoxanthin from brown algae (Sargassum filipendula) from Padike village, Talango district, Sumenep regency, Madura islands, using nuclear magnetic resonance (NMR)

Thallus color on brown algae (S. filipendula) is influenced by the different types of pigments and pigment content therein dominant. Therefore, brown algae (S. filipendula) that have been identified using column chromatography (CC), then analyzed by using High Performance Liquid Chromotography (HPLC), Fourier Transfom Infra-Red (FTIR), and Nuclear Magnetic Resonance (NMR) to determine the molecular structure of the pigment content and the dominantfucoxanthin from the stems, leaves and whole parts of plant from brown algae (S. filipendula). One to six, 7-62, 63-78, 79-135 types of pigment have been separated from S. filipendula respectively, in a single work step by Column Chromatography (CC) using a gradient elution of hexane:ethyl acetate (5:5 v/v) to obtain the separation of pigments. Identification of pigments made by order of polarity, Rf values, and structure of each pigment molecule compared tothe literature data that uses samples of brown algae (S. filipendula) and mobile phase were relatively the same. Type of brown algae was then explored for the stems, leaves, and whole part of plant, the leaves that have been proven to have highest fucoxanthin content (1.08 ± 0.080). FTIR identification of fucoxanthin crude extract showed the functional groups of OH, CH, C=O, C = C, CH2, C=O, COC and C=C trans substituted and isolated fucoxanthin had functional groups of OH, CH, allenic bond, C=O, C=C, CH2, COC and C=C trans substituted. HPLC analysis results showed the chromatogram peaks increase linearly and with spiking can be proved that the samples are identified as fucoxanthin. While NMR analysis showed the presence of O2 at 5.6 epoxide groups shown in the C5 and C6 atoms and the compounds were allene and acetate.