β-Glucan production of Saccharomyces cerevisiae by using malva nut juice production wastewater

Chanawanno, T.; Wasikadilok, N.; Khunphutthiraphi,T.; Mongkontanawat, N.; Phuangborisut, S.

β-Glucan production of Saccharomyces cerevisiae by using malva nut juice production wastewater

2018

Chanawanno, T. | Wasikadilok, N. | Khunphutthiraphi,T. | Mongkontanawat, N. | Phuangborisut, S.

Three yeast strains such as S. cerevisiae Angel®, S. cerevisiae TISTR 5020 and S. cerevisiae TISTR 5919 isolates were used in this study. β-Glucan production and the yield of three strainsof S. cerevisiae by using malva nut juice production wastewater were studied in order to reduce environmental pollution. The results showed that significantly higher %yield and β-glucan production was founded in S. cerevisiae TISTR 5919 (0.67 ± 0.01%w/v and 15.01%w/w;respectively) than that S. cerevisiae Angel® and S. cerevisiae TISTR 5020 (0.39±0.01%w/v, 12.69±2.62%w/w and 0.41±0.03%w/v, 8.33±1.26%w/w; respectively). Consequently, S. cerevisiae TISTR 5919 was selected to compare cell morphology by scanning electronmicroscopy (SEM) and the thickness of the cell wall by transmission electron microscopy (TEM) between cultured in YPD medium (control) and malva nut juice production wastewater. Our results found that multiple budding cell, thinner cell wall and abnormal cell were exhibited when yeast was cultivated in YPD medium supplement with malva nut juice production wastewater. Overall, the obtained results implied that the wastewater from malva nut juice production could be activate budding process and β-glucan formation, effected to the cell wall thinner and reduced the cell dry weight of yeast cell.

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