Comparative transcriptome analysis of papain-lite cystine protease-mediated resistance against Xanthomonas oryzae pv. oryzae in rice
2019
Nino, M.C. | Cho, Y.G.
High throughput transcriptome investigations of plant immunity highlight the complexity of gene networks leading to incompatible interaction with the pathogen. Accumulating findings implicate papain-like cysteine proteases (PLCPs) as central hub in plant defence. While diverse roles of PLCPs in different pathosystems have become more evident, information on gene networks and signaling pathways necessary to orchestrate downstream responses in unavailable. To understand the biological significance of cysteine protease against Xanthomonas oryzae pv. oryzae (Xoo), PLCP-overexpression and knockdown transgenic rice were generated. Pathogenicity test revealed the attenuation of Xoo K3a virulence in transgenic lines which is ascribed to high hydrogen peroxide and free salicylic acid accumulation. Next-generation sequencing of RNA from transgenic and wild type plants identified 1,597 combined differentially expressed genes, 1,269 of which were exclusively regulated in the transgenic libraries. It was found that PLCP aids rice to circumvent infection through extensive activation of transduction signal and transcription factors that orchestrate downstream responses including up-regulation of multiply pathogenesis-related proteins and biosynthesis of secondary metabolites.
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