Biological activity and chemical composition of autochthonous mushroom species Coprinus comatus (O.F. Müll.) Pers. Gray, 1797 and Coprinellus truncorum (Scop.) Redhead, Vilgalys & Monclavo, 2001 | Биолошка активност и хемијски састав аутохтоних врста гљива Coprinus comatus (O. F. Müll.) Pers. Gray, 1797 и Coprinellus truncorum (Scop.) Redhead, Vilgalys & Monclavo, 2001

The biological activity of extracts of basidiocarps (fruiting bodies) and submerged cultures (mycelium and filtrate) of autochthonous mushroom species Coprinus comatus and Coprinellus truncorum was examined. Furthermore, the metabolism of phosphate of mycelia of both types was studied using nuclear magnetic resonance spectros-copy ( 31 R NMR), the influence of vanadium on phosphate metabolism and the identification of vanadate oxidation states present in the mycelia cell ( 51 V NMR). The antiradical and antioxidant activity of methanolic, ethanolic and water fungal extracts was determined. Extracts of submerged cultures achieved the best anti- radical activity while fruit body extracts showed the best antioxidant activity. Extracts of submerged cultures also highlighted in terms of antibacterial activity, where the chloroform extract of the submerged culture C. comatus showed as the most potent. Also, the ethanolic extract of the submerged culture of C. comatus was found to be most relevant in anti-acetylcholinesterase activity compared with the conventional donepezil drug. The influence of extracts on the viability of cell lines HepG2 (human hepatocytes cells) and Rin-5F (ß pancreatic cells of the rat) was also examined. Spectrophotometric methods determined the total con-tent of phenol and flavonoids in most of the analyzed extracts. The LC/MS identification and quantification of phenolic acids revealed the difference between the phenolic compounds present in the fruiting body, mycelium, and the submerged culture filtrate. Extracts of submerged cultures record a greater number and higher content of compounds. The total content of proteins determined only in water extracts and the total content of carbohydrates in poly-saccharide extracts. Using the Fourier infrared spectro-scopic method (FTIR), the links between the sugar pre-sent in the polysaccharide extracts were detected, and planar chromatography showed that the extracts of the fruiting body and the filtrate of type C. truncorum, as well as the extract of the fruiting body of the species C. comatus, contain a large amount of D-glucose, while the extract of the C. truncorum mycelia and mycelia of C. comatus, contain the most galactose. GC-MS identification and quantification of fatty acids indicated a significant presence of linoleic acid in both species, while qualitative and quantitative elemental analysis (AAS) has determined a higher content of potas-sium and iron in the analyzed samples. Since there is no data in the literature for the autochtho-nous species C. truncorum, the studies on its chemical composition can be considered advanced аs innovative. A comparative review of the biological activity and the chemical composition of the extracts of the fruiting body and mycelia and filtrates of medium of submerged cultures indicated that the extracts were analyzed by sources of bioactive substances with medical potential, and the submerged cultures of these mushrooms are attractive candidates for biotechnological research.