Effect of aluminum on the growth of the in vitro culture tissues of the date
Madi Waheed Al-Mayahi, Ahmed
This study was carried out to evaluate the effect of different concentrations of aluminum: (25, 50 and 100 mg l–1 of aluminum chloride AlCl3 . 6H2O) on the enhancement of shoot multiplication, phytochemicals, as well as, antioxidant enzyme activity of in vitro cultures of the date palm cv. Um-Aldehin. The addition of aluminum in a concentration of 50 mg l–1 to the medium exhibited most effects on shoots regeneration and this substance also increased the number of shoots per a jar (73.34% and 8.2 shoots/jar, respectively), compared to the other concentrations, including the control treatment. A biochemical analysis of date palm shoots revealed that the high concentration of aluminum chloride (100 mg l–1) led to a significant accumulation of the total proline content, and to a high activity of ascorbate peroxidase, as well as a significant reduction in nitrogen, phosphorus, calcium, magnesium and chlorophyll, compared to the concentration of 50 mg l–1 for which there were recorded the highest contents of the mentioned elements, together with the highest content of chlorophyll in leaves.
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