??????????????????????????????????????????????????????????????????????????????????????????????????????????? | Genetic diversity of pandan-like fragrant cucumbers as assessed by SSR markers
2021
Kularb Laosatit(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy) | Akkarachai Somkul(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Horticulture. Tropical Vegetable Research Center) | Prakit Somta(Kasetsart University. Kamphaeng Saen Campus, Nakhon Pathom (Thailand). Faculty of Agriculture at Kamphaeng Saen. Department of Agronomy)
Thailand is a part of the center of diversity of cucumber (Cucumis sativus L.). Recently, a special type of cucumber, pandan-like fragrant cucumber, has been discoverred in Thailand. Pandan-like fragrance is a value-added trait. The fragrant cucumber is important and interesting as gene source in breeding new cucumber cultivar(s). However, at present, diversity and population structure of fragrant cucumbers are not known. The objective of this study was to assess genetic diversity and origin of the fragrant cucumbers. A set of cucumber germplasm of 96 accessions comprising 7 fragrant and 89 non-fragrant accessions were analyzed with 10 simple sequence repeat (SSR) markers locating on 7 chromosomes of cucumber. SSR analysis revealed that the markers detected 36 alleles in total with the number of alleles per marker ranging from 2 (SSR02895) to 6 (CS-SSR3) and an average of 3.6. Allelic richness between the fragrant and non-fragrant accessions was comparable, being 27.78 and 23.43, respectively. Overall gene diversity (HE) was moderate, being 0.52. HE between the fragrant and non-fragrant accessions was comparable, being 0.37 and 0.52, respectively. Neighbor-joining, principal component and STRUCTURE analyses generally showed that fragrant accessions were genetically different and were clustered into two groups with each group mingled with non-fragrant accessions. All together these results revealed that the fragrant cucumbers possess moderate genetic diversity and were different from non-fragrant cucumber. The results also suggested that the fragrant cucumbers have two different origins. The findings in this study are useful for conservation and utilization of fragrant cucumbers in breeding new cultivar(s)
Показать больше [+] Меньше [-]Ключевые слова АГРОВОК
Библиографическая информация
Эту запись предоставил Kasetsart University