Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols
2017
GOLUNSKI, SIMONE | SILVA, MARCELI F. | MARQUES, CAMILA T. | ROSSETO, VANUSA | KAIZER, ROSILENE R. | MOSSI, ALTEMIR J. | RIGO, DIANE | DALLAGO, ROGÉRIO M. | DI LUCCIO, MARCO | TREICHEL, HELEN
ABSTRACT The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in the crude extract on the purification yield and purification factor were evaluated. Precipitation caused an activation of enzyme and allowed purification factors up to 2.4-fold for both alcohols. The purification factor was affected positively by the modification of the ionic strength of the medium to 0.5 mol.L-1 NaCl before precipitation with the alcohol (n-propyl or iso-propyl). A purification factor of 4.8-fold and an enzyme yield of 78.1 % could be achieved by the addition of 0.5 mol.L-1 of NaCl to the crude extract, followed by the precipitation with 50 % (v/v) of n-propyl alcohol, added at a flow rate of 19.9 mL/min.
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