Cytokinin Dehydrogenase activity in primary roots and characterization of primary metabolites from leaves and rootlets of Ricinus Communis
Marques, Maria Elizabeth da Costa(Universidade Federal de Sergipe Programa de Pós-graduação em Biotecnologia de Recursos Naturais) | Freitas, Beatriz Tupinambá(Universidade Federal de Sergipe Departamento de Fisiologia) | Guedes, Tarciana Oliveira(Universidade Regional do Cariri Programa de Pós-Graduação em Bioprospecção Molecular) | Fernandes, Roberta Pereira Miranda(Universidade Federal de Sergipe Programa de Pós-graduação em Biotecnologia de Recursos Naturais ,Universidade Federal de Sergipe Departamento de Fisiologia)
The aim of this study was to determine the activity of cytokinin dehydrogenase (CKX) and to measure other biochemical components in the primary leaves and radicles of castor seedlings (BRS Energia) in the initial phase of growth. The crude protein extract obtained after a 1-h extraction from the root tissues of seedlings showed no detectable CKX enzymatic activity when incubated with the substrate isopentenyl adenine for 1 h. However, after precipitation with ammonium sulfate at 70% saturation, the pellet showed CKX activity. The peroxidase enzyme activity was higher in the leaves than in the radicles. The total and reducing sugar content was 1.5 times higher in the leaves than in the radicles. The amino acid and protein contents were 6.4 and 9.2 times higher in the leaves than in the radicles, respectively.
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